Chimeric Antigen Receptor T-Cell Therapy Improvements


Cellectis Publishes Novel Methods to Improve the Clinical Use of Chimeric Antigen Receptor T-Cell Therapy in Scientific Reports

CubiCAR, a Next Generation CAR, Allows for the Purification, Detection and Efficient Elimination of CAR T-Cells

Cellectis T-Cell Therapy Research (Alternext: ALCLS; Nasdaq: CLLS), a biopharmaceutical company focused on developing immunotherapies based on gene edited CAR T-cells (UCART), today announced the publication of a study in Scientific Reports, a Nature Publishing Group journal, describing the development of the CubiCAR, an all-in-one Chimeric Antigen Receptor (CAR) architecture with an embedded multi-functional tag for purification, detection and elimination of CAR T-cells. This added versatility has the potential to streamline the manufacturing of CAR T-cells to allow their tracking and efficiently eliminate CAR T-cells in clinical settings.

“The power and novelty of this technology lies in the integration of these multiple functions in one unique CAR molecule. To identify an optimal CAR architecture, we selected 15 different CAR constructs and evaluated their ability to enable T-cell depletion and promote tumor eradication,” said Julien Valton, Ph.D., Innovation Team Leader of Cellectis. “The development of this novel architecture was in collaboration with our colleagues at Allogene.”

“The CubiCAR architecture represents a major step forward in the development of CAR-T therapies in the treatment of various cancers,” added Philippe Duchateau, Ph.D., Chief Scientific Officer of Cellectis. “Not only is the CubiCAR architecture an integrated, compact safeguard allowing for the fast and efficient depletion of CAR T-cells, it is also compatible with multiple scFvs that are designed against different targets, which gives it the unique potential to make CAR T-cell immunotherapies safer.”

“The transformational impact of autologous CAR T therapy for the treatment of hematologic cancers has been firmly established. We plan to evaluate this CubiCAR approach and other novel CAR T engineering developed in partnership with our colleagues at Cellectis across our extensive CAR T pipeline,” said Barbra Sasu, Ph.D., Chief Scientific Officer of Allogene. “As we look to the future of allogeneic cell therapy, we can apply the scientific understanding gained from earlier therapies to the next wave of innovation to develop best-in-class allogeneic cell therapy.”

Julien Valton, Ph.D., Innovation Team Leader, Cellular Engineering & Adoptive CAR T-Cell Immunotherapy

Dr. Julien Valton obtained his Ph.D. at the University Joseph Fourier in Grenoble, France, where he was trained as an enzymologist. He then joined the Yale School of Medicine to apply his knowledge to therapeutic research by investigating the mechanism of inhibition of receptor tyrosine kinases that are involved in the development of gastrointestinal cancer. In 2009, he moved a step further into the field of applied science by joining the Innovation Department of Cellectis, where he actively participated in using and improving TALEN® gene editing technology for targeted gene therapy and genome engineering. He is now using TALEN® along with protein engineering techniques to develop the next-generation CAR T-cells to treat different malignancies.

A Versatile Safeguard for Chimeric Antigen Receptor T-Cell Immunotherapies

Julien Valton1, Valerie Guyot2, Bijan Boldajipour4, Cesar Sommer3, Thomas Pertel3 Alexandre Juillerat1, Aymeric Duclert2, Barbra Johnson Sasu3, Philippe Duchateau2 and Laurent Poirot2

1 Cellectis, Inc., 430E, 29th Street, NYC, NY 10016, USA;
2 Cellectis S.A., 8 rue de la Croix Jarry, 75013 Paris, France;
3 Allogene Therapeutics, 270 Littlefield Ave., South San Francisco, CA 94080, USA;
4 Pfizer Inc., 230 E Grand Ave, South San Francisco, CA 94080, USA.